FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763116

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763116
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2372195
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG4250.043.1384662
TCGCGTT555.2782612E-542.7362445
TACTCGG4750.039.5872573
CGGAGGT7150.032.871267
GCTACTC12650.032.332261
GGCGGAG7900.032.1333275
AGGCGGA8600.028.9711974
CCGAGTA4600.028.61472
TTGGGAG29800.028.2356876
GCGCGTT505.322438E-528.2035412-13
CTCGGGA6850.028.1372935
GCGGAGG8150.027.6850436
CGAGTAG4650.026.285093
GGAGGCT36000.025.9843679
ACTCGGG7800.025.9156974
GGAGGTT21350.025.7596768
GCTGGGA39400.025.650979
GGGAGGC39000.024.226128
TCGGGAG7400.024.1386746
CCCAAAG32100.024.0185151
Produced by FastQC (version 0.11.5)