FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763117

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763117
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2379549
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTAGC15400.043.332134
CGCGTGA704.6414116E-640.280573
CGAGTAG6950.039.2180143
AGTAGCT27550.037.8681225
CCGAGTA7500.036.9903762
GCTGGGA62750.036.9211859
CTACTCG5550.036.4312022
TAGCTGG31550.035.8971637
GCTACTC14100.035.712391
CCCAAAG40900.035.669141
GTAGCTG30300.035.2067266
TTGGGAG42350.033.844566
AAAGTGC32950.033.8014534
CCAAAGT37550.032.1826062
TACTCGG6800.031.7900583
AAGTGCT35400.031.5948375
AGTGCTG37800.030.8320436
CCCGAGT10200.030.4507541
GGAGGCT48150.030.3533429
CGGAGGT10550.029.844537
Produced by FastQC (version 0.11.5)