FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763119

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763119
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2428477
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGAG757.375449E-637.6529581
TACGCGT250.001702086337.60216562-63
CGGAGGT7900.027.9581667
TACTCGG5100.027.6432463
AGGCGGA7900.027.3633124
TTCGCGC350.00884028726.85620330-31
CGCACTA700.00884387126.8534494
CTACTCG4950.026.6008132
GCTGGGA37600.026.4963429
GGCGGAG8450.026.1384055
TAGCTGG22600.024.9523077
GCTACTC12100.024.8945141
GTAGCTG20550.024.697326
GCGGAGG8650.024.4474956
CTCGGGA6450.024.0431965
TTGGGAG31800.023.9400926
GGAGGTT19500.023.8582448
CGCGAGT1000.001744892823.5330961
GGAGGCT31950.023.2393599
AGTAGCT20250.023.2066735
Produced by FastQC (version 0.11.5)