FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763123

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763123
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3012584
Sequences flagged as poor quality0
Sequence length35-351
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGTC5402.9734126E-4116.07135310-319
TATAGCG2554.3365153E-4103.02617340-345
CACTACG3659.6635736E-486.33305310-319
CGTAGGT3300.003037480279.61113340-345
ATCCCGG4900.001427828878.241806260-269
ACGACAA5500.003662103361.70386320-329
CGTCAGT4350.00392450460.641727220-229
CGCAAGT3800.00869167859.304718320-329
CTACGGG3850.00957559958.534527320-329
ACCCGGC9453.274181E-1154.778423290-299
GCGATTG3202.702153E-454.62138290-299
AACCCGT3452.8045135E-454.210773340-345
CGACAAA4350.007051750652.297638320-329
TTCCGGG9002.2763129E-450.079536320-329
TTAGGCG2602.530217E-549.151905260-269
AGTAGCT40500.043.8318825
CGAGTAG10850.043.1388783
GTAGCTG41200.042.3944476
CCGAGTA11750.041.795822
GAGTAGC27550.041.6446464
Produced by FastQC (version 0.11.5)