FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763125

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763125
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2807879
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG6800.031.7893473
GAGTAGC17050.030.8692684
CCGAGTA7400.030.5014652
CGGAGGT7100.029.7842647
GCTGGGA53000.029.703099
CCCAAAG43600.029.2495481
AGTAGCT26600.028.9730955
TAGCTGG28900.028.2933317
CTACTCG6350.028.1398032
CTCGCGT1057.43291E-526.8531134
GTAGCTG29350.025.6179626
TACTCGG6250.025.5641633
CCCGAGT10000.025.4114881
AAAGTGC37250.025.2311124
GGAGGTT24600.025.2157298
GCTACTC16200.024.9815711
GTGCTGG36900.024.8336737
CCAAAGT44600.024.7767412
TTGGGAG38850.024.5548746
GCGGAGG10600.024.3831336
Produced by FastQC (version 0.11.5)