FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763126

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763126
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2807879
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG6900.045.6445123
CTACTCG7000.042.9786262
CCGAGTA7600.040.20412
TACTCGG7850.038.9230233
GAGTAGC17950.034.0440944
CCCGAGT10350.033.610691
CGGAGGT9650.033.122367
TCGGGAG9800.032.6159676
GCTGGGA61300.032.0525979
AGTAGCT30050.031.7546025
TAGCTGG33250.031.524847
TGCGACG902.5929094E-531.3374655
GGAGGCT47050.030.4710669
CTCGGGA10700.030.3124075
CCCAAAG44200.029.8861621
GTAGCTG31850.029.3691276
TTGGGAG43700.028.6119586
ACTCGGG11800.027.885534
AAAGTGC36550.026.7509274
GGGAGGC55250.026.5442058
Produced by FastQC (version 0.11.5)