FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763127

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763127
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3682887
Sequences flagged as poor quality0
Sequence length35-351
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTATA3501.6218957E-5132.6818340-345
TCGCGAC1150.00114760160.454166280-289
CCTAGCG2953.1773798E-656.242874300-309
ATACCGG3650.00560372749.77475310-319
CGAGTAG14800.049.5181163
AGTAGCT54200.047.218125
CCGAGTA15200.047.0842972
TAGACGG4952.253195E-646.907707340-345
GAGTAGC36250.046.370864
AATCGCG1150.00114760145.63501250-259
GCTGGGA122000.044.9103439
GTCGATA2450.007210339444.16662330-339
GTAGCTG56900.042.8308836
AAGTGCT72400.042.1770365
TTGGGAG86450.041.7812966
TTGCCGG5601.12148344E-441.463062340-345
TACTCGG13800.040.4620133
TAGCTGG62450.040.2352457
CAACGGA4803.1298827E-439.464622240-249
CTACTCG13150.039.3802832
Produced by FastQC (version 0.11.5)