FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763129

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763129
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2895756
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG5000.030.076363
CGAGTAG5550.028.7893073
CCGAGTA6950.027.7357142
CTACTCG5450.026.7427142
CCCGAGT8850.025.5192831
CGTCGAG1501.1525699E-625.0939621
CCCGTTA1351.4461926E-524.3969061
CCCAAAG39750.024.1470181
CTCGGGA7100.023.8281025
GCTGGGA44650.023.7865949
GCTACTC12350.023.6208361
TAGCTGG27200.023.4971567
CGGAGGT6650.023.3204847
CGCCGGG3650.023.1752765
TCGGGAG6900.023.1566186
AGTAGCT24750.022.9749975
GAGTAGC15750.022.3782424
TAGGACG1703.4584664E-622.1149716
GGAGGCT36800.021.9647339
CCTATAG7950.021.8980331
Produced by FastQC (version 0.11.5)