FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763130

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763130
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2895756
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG5300.031.9284482
CTCGCGT1501.0695658E-931.337184
TTGGGAG39950.029.5320176
GCTACTC15650.029.4369561
CGAGTAG5700.028.8631943
CCGAGTA6150.028.2798962
GGGAGGC48850.028.0004238
TCGCGTT856.813717E-427.6504525
CGGAGGT9000.027.1579517
GGAGGCT43450.027.0449629
TCGACGG1057.4192045E-526.8604373
ACGACGC350.00883660326.85858390-91
CCCGAGT8950.026.787261
GCTGGGA48050.026.6079659
CCGCGAT909.5143635E-426.1143172
TACTCGG6650.025.446733
TGGGAGG52450.025.3616267
CCCAAAG41800.025.1914331
GAGTAGC13850.025.1149964
GGCGGAG11850.024.990415
Produced by FastQC (version 0.11.5)