FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763133

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763133
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2843776
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTT450.00101497441.7713745
TACTCGG5000.029.135533
CTACTCG4700.028.0134372
GCTGGGA45400.027.0156789
CCGAGTA5950.026.0797372
CGAGTAG5350.025.4727293
CCCAAAG40400.025.0393581
TCGGGAG7150.024.3179476
CCCGAGT8450.023.9429591
ACCGCGC2602.7284841E-1123.5253491
CCGGGTT4850.023.2541668
GGGAGGC36300.022.913848
CGGAGGT7650.022.7285397
TAGCTGG24150.022.5721057
GCTACTC11050.022.5673051
CGGTGCG1050.00233841922.377526
GTGCTGG32350.021.6442837
GGAGGCT34800.021.4708469
GTACCGG1100.003052133621.373892
CGGGTTC5250.020.5873189
Produced by FastQC (version 0.11.5)