FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763134

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763134
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2843776
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG4850.039.741562
TACTCGG5350.038.6635483
CTCGGGA7300.033.4869585
TCGGGAG8400.031.8994466
GCTGGGA46700.031.6054619
CGAGTAG5100.031.3409083
ACGCGAT650.00614840828.9300693
TTGGGAG37200.028.054186
GCTACTC14300.027.6160371
CCGAGTA6000.027.4232942
GGAGGCT41900.027.3731759
GGGAGGC46300.027.3094568
CCCGAGT7450.027.135031
TAGCTGG24050.025.994317
GAGTAGC13050.025.9368464
GGCGGAG11450.024.6343425
AGTAGCT23300.024.6149585
CCGATAG1151.3753182E-424.5285321
ACTCGGG9050.023.894864
GGAGGTT26250.023.8157398
Produced by FastQC (version 0.11.5)