FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763136

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763136
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2553863
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA4400.029.9144362
TTCGGCG650.006150183728.9282475
TACTCGG5800.028.367143
GCTACTC14000.027.1993181
CGAGTAG4550.026.8619423
CTACTCG5800.025.935672
CCCGAGT6600.024.9301621
TCGGGAG7250.024.6384036
GGAGGCT34000.024.6093169
GAGTAGC12100.024.0869484
GGGAGGC36100.023.828818
TAGCTGG24250.023.64827
AGTAGCT21700.023.6125145
CTCGGGA7600.022.8856685
GCTGGGA41800.022.8285459
CGGAGGT7750.022.4414027
TTGGGAG31550.022.0510546
CCGGGTT4350.021.6122138
TGGGAGG41850.021.5653117
GTAGCTG23650.021.466426
Produced by FastQC (version 0.11.5)