FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763138

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763138
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2202108
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG5250.045.6655043
CTACTCG5200.043.3935472
CGAGTAG5100.040.5564463
CGGAGGT7400.037.4764337
CCGAGTA6100.034.679272
GCTACTC12850.034.0241131
GAGTAGC13650.032.715874
TCGGGAG7650.032.5665746
AGGCGGA10100.031.648644
GGCGGAG10350.031.3383625
GCTGGGA45850.031.2678819
GGAGGCT36250.030.7311139
CCCAAAG36250.030.3469351
TTGGGAG33500.030.1683336
CTCGGGA7450.029.6557625
CCCGAGT7800.029.533061
GTGCTGG29750.029.3875897
CCGGTAA804.7765288E-429.3810482
GGGAGGC43100.028.682468
AGTAGCT21050.028.3608445
Produced by FastQC (version 0.11.5)