FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763140

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763140
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2119668
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACCG408.023826E-658.7653543
TACTCGG4550.044.4291883
CTACTCG4500.039.6992572
CCGAGTA5150.038.3401152
CGAGTAG5100.037.794193
CCCGAGT7100.032.44511
AGTAGCT19800.031.8156415
TAGCTGG22350.030.9157267
CTCGGGA6700.030.873015
GCTACTC9950.030.7115421
CGGAGGT7200.030.6835047
GCTGGGA40650.030.4112879
GAGTAGC12100.030.3054374
CCCAAAG33900.029.954731
TTGGGAG33200.029.5908936
GGAGGCT35050.029.369449
GTAGCTG22700.028.5761096
GGGAGGC41150.028.5568168
AGGCGGA9200.028.105174
TCGGGAG8250.027.9184956
Produced by FastQC (version 0.11.5)