FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763141

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763141
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2352057
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA4800.039.193322
CGAGTAG4500.035.5065773
TACTCGG4100.033.2396553
CGGAGGT8650.032.5969927
CCCGAGT6850.031.615141
ACGACCG600.00416446331.3293296
GCTACTC11150.030.8230671
CTACTCG4750.030.694562
GCTGGGA42850.030.3788499
GAGTAGC12900.030.2364464
GCCGGTA1303.3378456E-728.9193828
AGTAGCT20700.027.923975
TTGGGAG32150.027.480166
GGAGGTT22250.027.4571658
CCCAAAG36350.027.0688441
AGGCGGA9550.027.0646044
CGTCGCG700.00884337726.853719
TAGCTGG23150.026.7957127
GGAGGCT36050.026.5929979
GGCGGAG9950.026.448885
Produced by FastQC (version 0.11.5)