FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763143

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763143
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2090978
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG3650.032.2166982
CGGAGGT4800.028.3933477
CCCGAGT5600.027.7377821
TACTCGG4200.026.8547923
CTCGGGA5100.025.8016625
CGAGTAG4250.025.4330673
GCTGGGA33200.025.3381429
CCGAGTA4650.024.276842
GCTACTC10550.023.646631
CGAACGT500.001758879223.50131648-49
GGAGGTT16250.023.1364368
ATAGCGC1252.4257919E-422.5580254
GCGGAGG5800.021.8773966
TAGCTGG19250.021.7279687
GCGTAAC653.162424E-421.69144880-81
GGCGGAG6550.021.5248345
GGGAGGC28800.021.3766028
AAACGCG550.003062432221.36176720-21
GGAGGCT28300.021.256099
TTGGGAG27850.020.9245596
Produced by FastQC (version 0.11.5)