FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763144

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763144
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2090978
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTACG352.936888E-453.7186623
TCGCGTT555.282049E-542.730755
ACGTTCG456.193277E-736.55721728-29
CTCGGGA5900.031.8675
TCGGGAG5900.031.8654776
GCTACTC9950.031.6538491
TACTCGG5050.030.7153723
CTACTCG4800.030.3566362
GGGAGGC33150.028.9240478
CGAGTAG3600.028.724563
CCGAGTA3950.028.5592862
CTCGCGT1005.3226344E-528.2022974
GCTGGGA36800.027.7156059
ACTCGCG856.814725E-427.6493113
TTGGGAG29550.027.0398246
TCGACGG700.00883413826.8593313
CGGAGGT5900.026.2890177
GGAGGCT33800.026.2819489
CGCGTTG909.5184287E-426.1119886
GAGTAGC10650.026.0396794
Produced by FastQC (version 0.11.5)