FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763148

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763148
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4208548
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA13900.028.7036232
CTACTCG11950.028.2811582
CGAGTAG13250.026.5685393
GCTACTC29850.026.357761
TACTCGG12200.025.777293
CCCGAGT18250.024.7824781
GCTAGCG1401.8974682E-523.5562631
GAGTAGC29250.023.2682864
GCTGGGA91750.023.1625969
GTCGCCG3750.022.7094194
CTAGCGA1252.4463766E-422.5306552
TCGGGAG16450.021.3859526
AGTAGCT49050.021.1480525
GTAGCTG49100.021.1127076
TAGCTGG55450.020.6427677
CCCAAAG71500.020.4264091
CGCCGTA1404.986762E-420.2762694
CGGAGGT13750.019.7881377
CTCGGGA15850.019.5448255
AAAGTGC59500.018.9328754
Produced by FastQC (version 0.11.5)