FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763149

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763149
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3973075
Sequences flagged as poor quality0
Sequence length35-351
%GC47

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGTC3956.2260294E-496.439285330-339
CGAGTAG28850.071.916623
AGTAGCT108800.070.773945
GTAGCTG110550.069.3611156
CCGAGTA30900.066.9810642
CGAATCG1905.053952E-466.830734330-339
GAGTAGC74200.064.41294
TAGCTGG123100.063.720577
GCTGGGA225000.063.2015049
AAGTGCT110350.061.2741935
TACTCGG26750.060.4802173
CTACTCG26650.059.811992
CCCGAGT37250.057.7830961
TTGGGAG149900.057.2850466
AGTGCTG120050.054.674296
AAAGTGC121700.053.8852274
GGAGGCT149850.053.8683329
GCTACTC58950.053.7197531
GCACTTT125000.052.15291
CAAGTAG48700.051.859723
Produced by FastQC (version 0.11.5)