FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763150

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763150
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3973075
Sequences flagged as poor quality0
Sequence length35-251
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGCT102350.073.772325
CGAGTAG28650.073.2848053
GAGTAGC68450.070.320424
GTAGCTG106700.070.284626
CCGAGTA30450.068.762362
CTACTCG27950.066.333442
TAGCTGG121200.064.196567
TACTCGG29050.063.820063
GCTGGGA229200.063.3092589
TTGGGAG162800.062.2217526
AAGTGCT112400.061.712545
GCTACTC69250.061.2204171
CCCGAGT36150.058.5567171
GGAGGCT177000.057.7362029
GCACTTT126300.057.6164131
ACCCGCA8209.488633E-657.100918245
TTTGGGA143000.056.564145
AAAGTGC122050.056.353984
ACTTTGG131400.056.0814553
AGTGCTG122750.055.9518476
Produced by FastQC (version 0.11.5)