FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763152

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763152
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3846860
Sequences flagged as poor quality0
Sequence length35-251
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG30500.077.94143
AGTAGCT112650.075.304265
CCGAGTA32250.073.904412
GTAGCTG114600.073.237256
GAGTAGC74600.071.4723364
TTGGGAG168500.067.48226
CTACTCG31450.066.994912
AAGTGCT117150.066.643895
GCTGGGA244900.066.201059
TAGCTGG130200.065.653137
CCCGAGT37850.065.0758741
TACTCGG32700.064.995823
GCTACTC75300.064.358461
GGAGGCT183000.063.055869
AGTGCTG127150.061.0349856
TTTGGGA148050.060.3271835
GCACTTT134150.060.0403141
TCGGGAG39900.058.1657076
AAAGTGC135050.057.7197044
ACTTTGG137500.057.6740723
Produced by FastQC (version 0.11.5)