FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763154

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763154
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3797758
Sequences flagged as poor quality0
Sequence length35-251
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG19600.071.233583
AGTAGCT81350.065.089735
CCGAGTA21650.064.222712
GTAGCTG82050.063.583036
GAGTAGC51950.063.00674
CTACTCG21700.060.870992
GCTGGGA174650.059.8550579
TACTCGG21700.059.5345343
TTGGGAG129200.057.050696
GCTACTC53100.056.223921
TAGCTGG95300.056.025347
GTCGCTT5550.005519349655.638332245
AAGTGCT97900.055.2698175
GGAGGCT135500.055.2651679
CCCGAGT25750.054.8127441
AGTGCTG104400.050.636696
CTCGGGA27500.050.348795
AAAGTGC107350.049.8647654
GCACTTT106700.049.8091551
TCGGGAG30300.049.6850476
Produced by FastQC (version 0.11.5)