FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763155

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763155
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4197408
Sequences flagged as poor quality0
Sequence length35-351
%GC46

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGTCG2250.002341611570.580315340-345
AGTAGCT92750.060.9546055
CGAGTAG23850.060.0853773
CCGAGTA25200.058.8302732
GAGTAGC60900.058.4799274
GTAGCTG96150.057.3581856
GCTGGGA189350.056.636499
CCCGAGT28550.055.1716651
TAGCTGG105950.054.1998637
TACTCGG22200.052.294633
CTACTCG22550.052.0583652
AAGTGCT105550.051.672225
TTGGGAG133900.050.3451356
AGTGCTG111650.047.8804746
CCCAAAG135900.047.2982181
GGAGGCT131350.047.074819
GCTACTC55200.046.7540631
AAAGTGC116100.046.195564
CCAAAGT126950.046.1877022
TTCGCGG3103.5560006E-644.88571320-329
Produced by FastQC (version 0.11.5)