FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763156

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763156
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4197408
Sequences flagged as poor quality0
Sequence length35-251
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTAAA4300.002024719371.66607245
CGAGTAG25750.067.2360843
CCGAGTA26200.065.643072
AGTAGCT97550.064.6646355
GAGTAGC61950.064.094544
GTAGCTG97650.064.028336
CTACTCG27950.061.5330432
TACTCGG28500.059.7359163
TAGCTGG106450.059.6071557
GCTGGGA197950.059.5665789
GCTACTC61200.057.5845871
CCCGAGT30350.056.8195651
TTGGGAG145600.056.767526
AAGTGCT103850.056.573785
GGAGGCT152800.056.063529
TCGGGAG34700.052.027156
TTTGGGA130900.050.702825
AGTGCTG114000.050.4435276
AAAGTGC115900.050.393094
GTGCTGG117500.048.895947
Produced by FastQC (version 0.11.5)