FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763157

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763157
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4878372
Sequences flagged as poor quality0
Sequence length35-351
%GC45

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCGTA1800.008237272598.38564280-289
CGAGTAG29450.061.176413
AGTAGCT108550.059.523695
GTAGCTG109000.058.989286
CCGAGTA31250.058.9320222
GCTGGGA207450.056.3587279
GAGTAGC72900.055.4729924
TAGCTGG121600.054.409997
CCCGAGT37850.051.96761
TTGGGAG155300.048.949366
GGAGGCT138500.048.0974969
AAGTGCT121150.045.4694825
TACTCGG22750.043.299883
AGTGCTG124950.043.2713626
GGGAGGC199850.042.33788
GTGCTGG128100.041.5922137
TTTGGGA153350.041.4581345
CGGAGGT29750.040.9102557
AAAGTGC135500.040.7497334
TCGGGAG28300.040.7271426
Produced by FastQC (version 0.11.5)