FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763158

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763158
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4878372
Sequences flagged as poor quality0
Sequence length35-251
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG27100.068.901593
CCGAGTA28000.067.784422
AGTAGCT104750.062.2730065
GAGTAGC69100.061.0665864
GTAGCTG107150.060.9684526
CTACTCG30150.058.265052
TACTCGG30900.057.844153
CCCGAGT34050.057.4984441
TTGGGAG168250.056.4900056
GCTGGGA219850.056.478889
GGAGGCT167100.053.94879
TAGCTGG124900.053.6875767
GCTACTC71000.052.5485081
CTCGGGA34650.050.166365
TCGGGAG37650.050.0644076
AAGTGCT125400.048.932495
GCACTTT139900.048.2764131
CGGAGGT37300.047.2483527
GGGAGGC237350.047.215798
TTTGGGA162000.046.977045
Produced by FastQC (version 0.11.5)