FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763161

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763161
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3058656
Sequences flagged as poor quality0
Sequence length35-351
%GC49

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGCT103800.082.546355
GTAGCTG107550.078.237416
CGAGTAG30100.076.3779453
GCTGGGA216600.072.556669
CCGAGTA32250.071.9430162
GAGTAGC73400.071.372744
AAGTGCT103650.070.747155
TAGCTGG121150.070.6879357
AGTGCTG110350.065.606836
TTGGGAG141350.062.3771326
CCCGAGT37900.062.1562161
AAAGTGC118850.060.920944
AAGTAGC47800.060.8107034
CAAGTAG46250.060.4204333
GTGCTGG122650.060.1944667
GGAGGCT137350.059.6605079
CCAAGTA48050.059.0167242
TCGGGAG31050.058.8752636
CCAAAGT129700.058.5077172
GCACTTT113150.057.779821
Produced by FastQC (version 0.11.5)