FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763162

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763162
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3058656
Sequences flagged as poor quality0
Sequence length35-251
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGCT100100.085.588535
CGAGTAG28250.083.563223
GTAGCTG105700.081.0116356
GAGTAGC67100.078.7214664
CCGAGTA30850.078.519832
CGCACTT4751.5475525E-677.50613245
TAGCTGG117800.074.372377
GCTGGGA221500.072.491399
AAGTGCT104100.071.997555
CAAGTAG39250.071.450723
TTGGGAG148850.069.9431846
CCCGAGT36000.069.16221
CTACTCG31550.069.158552
GCTACTC71750.068.4561
AAGTAGC44400.066.217124
TACTCGG33000.065.7451253
TTTGGGA125400.065.617345
GGAGGCT165900.065.2917259
GCACTTT116050.063.7257921
AGTGCTG116650.063.5314066
Produced by FastQC (version 0.11.5)