FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763164

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763164
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2997624
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG11250.058.001373
CTACTCG11900.054.0460622
CCGAGTA11950.053.819932
CGTACCG352.9557623E-453.6497763
GAGTAGC28550.052.2873954
TACTCGG12750.051.5458643
GCTACTC29050.050.2869531
AGTAGCT46800.048.8477485
GCTGGGA98350.048.2708449
CCCAAAG67150.047.650071
AAAGTGC55400.047.5367054
CCCGAGT14000.046.7871441
GTAGCTG49000.046.233566
AAGTGCT58000.045.8089035
CCAAAGT62900.043.810472
AGTGCTG61350.043.2721186
TAGCTGG54250.043.061257
TTGGGAG78550.042.7536436
GGAGGCT81200.042.1163029
CAAAGTG64900.041.1569823
Produced by FastQC (version 0.11.5)