FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763170

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763170
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3600492
Sequences flagged as poor quality0
Sequence length35-251
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG22300.067.5397643
AGTAGCT82350.065.743185
CCGAGTA23800.064.506532
TACTCGG23550.064.201783
GTAGCTG86300.062.901266
CTACTCG24150.062.3676452
GAGTAGC53850.059.9338154
GCTACTC57100.057.7913741
TAACCCG3400.00492275557.276653235-239
GCTGGGA181100.057.135139
TAGCTGG99750.056.2937137
GGAGGCT134000.056.2214369
TTGGGAG129200.055.6905366
CCCGAGT28950.054.5762141
AAGTGCT94950.053.4667665
TAGAGCG5601.1238406E-452.70641245
TCGGGAG29950.052.2009936
CTCGGGA28950.052.0254485
ACTCGGG28950.050.4184844
GCACTTT103100.050.102311
Produced by FastQC (version 0.11.5)