FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763171

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763171
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2745777
Sequences flagged as poor quality0
Sequence length35-351
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGCT77450.075.409025
GTAGCTG78850.074.4810266
CGAGTAG23400.073.5331343
CCGAGTA24950.070.49692
GAGTAGC53300.069.5411764
TAGCTGG87250.069.052217
GCTGGGA162500.067.251889
AAGTGCT76450.064.7787865
TTGGGAG106750.061.191686
CCCGAGT30000.059.7468031
AGTGCTG85050.057.6032876
AAAGTGC88000.056.1364064
TTTGGGA97750.054.764325
GGAGGCT105400.054.648119
CAAGTAG32000.053.578383
CTACTCG16950.052.7951472
CCAAAGT97550.052.4472772
GTGCTGG92900.052.120797
CCCAAAG109450.051.6140981
CAAAGTG96000.051.5868573
Produced by FastQC (version 0.11.5)