FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763172

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763172
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2745777
Sequences flagged as poor quality0
Sequence length35-251
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGCT74750.074.490095
CGAGTAG19800.073.476933
GTAGCTG76800.071.710926
GAGTAGC48250.070.112924
CCGAGTA21050.069.6688162
CTACTCG22900.066.081682
TAGCTGG86850.065.102217
TACTCGG23100.064.750753
GCTGGGA160600.064.380979
GCTACTC51000.063.5423741
AAGTGCT76500.063.23915
TTGGGAG114350.063.1783686
CCCGAGT24000.062.2640531
GGAGGCT121850.060.5970239
GCACTTT88950.058.700291
TTTGGGA101000.057.617555
CAAGTAG32350.056.5310253
CACTTTG91600.056.4500852
AAAGTGC85050.055.9199644
ACTTTGG92700.055.780233
Produced by FastQC (version 0.11.5)