FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763174

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763174
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2958591
Sequences flagged as poor quality0
Sequence length35-251
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAAGCG2606.216015E-496.481865245
AGTAGCT89100.077.7444845
CGAGTAG24750.075.75523
GTAGCTG91000.074.4921956
CCGAGTA26000.073.504372
GAGTAGC59450.072.6086654
AAGTGCT92950.070.243545
GCTGGGA192700.068.797079
TTGGGAG142600.068.664696
CTACTCG28700.068.059762
TAGCTGG104150.067.28877
TACTCGG29250.067.192223
GCTACTC64850.065.988531
GCACTTT106350.065.0180051
GGAGGCT157900.064.572459
TTTGGGA122650.063.949485
AGTGCTG101050.063.0883266
CCCGAGT31200.061.99271
CAAGTAG35450.061.5629733
ACTTTGG114400.061.3414733
Produced by FastQC (version 0.11.5)