FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763177

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763177
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4674065
Sequences flagged as poor quality0
Sequence length35-351
%GC45

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG28950.064.543023
AGTAGCT110350.063.920845
GTAGCTG110500.062.3936166
CCGAGTA29850.062.183182
GCTGGGA216950.058.594499
GAGTAGC76350.057.599174
TAGCTGG128200.056.2618837
CCCGAGT35650.054.0029641
TTGGGAG158500.052.6271636
GGAGGCT148250.049.8657769
AAGTGCT122400.047.051045
CTACTCG25150.046.1938062
AGTGCTG124850.045.2352266
GGGAGGC211950.045.100518
TTTGGGA155700.044.1885645
TACTCGG25950.043.974273
GTGCTGG128850.043.768437
CGGAGGT31450.043.6638077
CAAGTAG51850.042.858343
GCACTTT139350.042.4542241
Produced by FastQC (version 0.11.5)