FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763178

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763178
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4674065
Sequences flagged as poor quality0
Sequence length35-251
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG26500.068.4973
CCGAGTA28300.065.471932
AGTAGCT104550.063.0783355
GTAGCTG106850.061.628116
TACTCGG30050.061.450083
GAGTAGC67400.061.224474
CTACTCG29650.059.3134082
TTGGGAG164300.057.7304276
GCTGGGA221200.056.9054159
CCCGAGT34050.055.2754631
GGAGGCT167900.054.5169039
CTCGGGA35400.054.114235
TAGCTGG127300.053.6545147
GCTACTC74450.053.09741
AAGTGCT124300.052.196835
TCGGGAG36950.051.815936
TTTGGGA155450.049.3332635
ACTCGGG36800.049.3252834
GCACTTT141250.048.709091
AGTGCTG128650.048.6478086
Produced by FastQC (version 0.11.5)