FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763179

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763179
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3331965
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG13750.054.086163
CCGAGTA14900.052.5044022
CTACTCG11450.051.4491732
TACTCGG12100.051.3476223
AGTAGCT53350.047.5538945
GAGTAGC33800.047.486424
GCTACTC23800.046.6021351
CCCGAGT18200.044.3444821
GTAGCTG57950.043.485136
GCTGGGA114200.043.4535379
CCCAAAG68300.043.3273121
AAAGTGC57100.042.9470524
TAGCTGG60250.042.2736327
AAGTGCT59700.042.180455
TCGCGAA450.001014369841.77663494
CCAAAGT63600.041.1994632
AGTGCTG63800.039.2767876
TTGGGAG74900.038.6031466
TCGGGAG17100.038.216256
CAAAGTG64750.038.091083
Produced by FastQC (version 0.11.5)