FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763181

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763181
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4373992
Sequences flagged as poor quality0
Sequence length35-351
%GC48

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGCT144050.078.830675
CGAGTAG41650.076.420173
GTAGCTG149050.075.551046
CCGAGTA45050.072.555482
GAGTAGC100550.071.8790054
TAGCTGG165800.068.7038047
GCTGGGA288350.068.452719
AAGTGCT137750.068.432155
TTGGGAG186350.063.462176
CCCGAGT53000.062.9611851
AGTGCTG147650.061.1705446
AAAGTGC158150.058.5072174
TTTGGGA168250.058.1288875
CAAGTAG60750.058.1090663
GGAGGCT190850.058.104129
CCAAAGT170200.057.5382162
CTACTCG33850.057.404982
GCACTTT153450.056.5063361
TACTCGG35200.056.0807153
AAGTAGC65050.055.750614
Produced by FastQC (version 0.11.5)