FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763185

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763185
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3995744
Sequences flagged as poor quality0
Sequence length35-351
%GC48

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGCT113050.072.885855
CGAGTAG30950.070.635883
GTAGCTG117100.070.181586
CCGAGTA33100.067.022512
GAGTAGC78050.066.750594
GCTGGGA227600.064.956359
ACGCTAG2750.00303218364.73448320-329
TAGCTGG129750.064.176527
AAGTGCT111550.062.7694855
CTACTCG25850.058.8820042
TTGGGAG148450.057.5980956
CCCGAGT39250.057.059151
TACTCGG25900.056.3515973
AGTGCTG123100.056.2170456
GGAGGCT152450.053.7727369
AAAGTGC128650.053.7082144
CAAGTAG49800.053.1736833
CCAAGTA52850.052.9367182
CCCAAAG154100.052.6879841
GTGCTGG131150.052.424997
Produced by FastQC (version 0.11.5)