FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763187

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763187
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3296045
Sequences flagged as poor quality0
Sequence length35-351
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGCT100650.077.198095
CGAGTAG28450.074.008293
GTAGCTG106250.073.880356
GAGTAGC66800.072.337944
CCGAGTA31350.069.1854552
TAGCTGG118900.067.978537
GCTGGGA210150.065.4383549
AAGTGCT95700.063.7976385
TTGGGAG133200.062.379766
CCCGAGT36200.059.8062671
CTACTCG23650.057.8120652
AGTGCTG104600.057.7822766
TTTGGGA120750.057.5065965
TACTCGG23550.057.2310643
GGAGGCT140100.056.539099
CAAGTAG44150.056.271993
TGAGTAG46550.055.6390083
CCAAGTA46100.055.27262
AAGTAGC47350.054.698964
GCTACTC56600.054.6280861
Produced by FastQC (version 0.11.5)