FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763188

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763188
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3296045
Sequences flagged as poor quality0
Sequence length35-251
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG27750.078.922933
AGTAGCT97700.078.00775
CCGAGTA27900.075.978962
GTAGCTG101550.073.788976
GAGTAGC65300.073.4625854
AAGTGCT99700.067.255585
GCTGGGA214300.067.230939
TAGCTGG116350.067.13137
CTACTCG28100.066.661432
TACTCGG28700.065.058273
TTGGGAG148200.065.013786
GCTACTC63700.064.792451
CCCGAGT32650.064.737671
GCACTTT113900.064.3046651
ACTTTGG115450.061.5387573
TAAGGCG3000.001497689361.327896245
AGTGCTG107600.061.2963336
TTTGGGA126950.061.005565
CACTTTG118400.060.803572
AAAGTGC110950.060.5418974
Produced by FastQC (version 0.11.5)