FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763189

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763189
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3759776
Sequences flagged as poor quality0
Sequence length35-351
%GC47

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCGTA1107.558608E-491.87134220-229
AGTAGCT90900.068.7975
CGAGTAG26550.065.341093
GTAGCTG94650.065.115786
GAGTAGC61650.063.048164
TAGCTGG104900.061.4583477
CCGAGTA29000.060.8827972
GCTGGGA190200.060.6548279
AAGTGCT96100.057.568185
CCCGAGT31750.056.994451
TTGGGAG128200.053.602416
CTACTCG20900.051.942882
AGTGCTG104700.051.699196
TACTCGG21700.050.5493973
AAAGTGC108700.050.5112344
CCAAAGT119950.049.926872
GGAGGCT127250.049.727619
CAAGTAG41200.049.197113
CCCAAAG131800.049.1640931
GTGCTGG112300.048.503077
Produced by FastQC (version 0.11.5)