FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763191

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763191
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3589885
Sequences flagged as poor quality0
Sequence length35-351
%GC48

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTACGC4108.7322056E-488.56778320-329
AGTAGCT103750.075.3763965
GACGGAT4700.001900948772.80966310-319
GTAGCTG108250.072.405826
CGAGTAG30550.070.0143363
GAGTAGC72750.068.658984
CCGAGTA32600.066.394142
TAGCTGG122500.065.71257
GCTGGGA213850.064.721669
AAGTGCT101300.059.4725885
CCCGAGT37600.058.2881771
TTGGGAG136650.057.671386
CTACTCG26200.057.1032942
TACTCGG26950.055.2611923
GGAGGCT145000.055.093569
CAAGTAG43550.054.0400623
TGAGTAG50600.054.0203173
AGTGCTG110550.053.0083926
GCTACTC58150.052.8917731
TCGGGAG31500.052.6805346
Produced by FastQC (version 0.11.5)