FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763193

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763193
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3782326
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG11550.050.93973
CCGAGTA12400.048.614932
GAGTAGC31000.043.576124
CTACTCG9400.040.582482
CCCGAGT14850.040.330041
TACTCGG9650.039.5080873
AGTAGCT51100.039.3310285
GCTGGGA99000.038.9719859
GTAGCTG52750.038.0549166
CCCAAAG70400.037.1097871
TAGCTGG56250.036.9270257
AAAGTGC57200.036.7000924
AAGTGCT59100.035.9184345
GCTACTC24150.035.5390361
TTGGGAG75000.034.0364276
GGAGGCT70000.033.970129
CCAAAGT66700.033.044672
CAAAGTG66850.032.3880463
AGTGCTG65800.031.865036
GTGCTGG66000.031.6143057
Produced by FastQC (version 0.11.5)