FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763194

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763194
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3782326
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG10600.053.590192
TACTCGG11250.052.1610153
CGAGTAG12800.049.5122153
CCGAGTA13950.046.441972
CCCGAGT17000.041.567171
GCTGGGA107350.041.1730849
GAGTAGC31650.040.3444444
TTGGGAG80700.039.7623866
CGGAGGT18700.039.3901947
TAGCTGG56450.039.229087
AGTAGCT52850.039.0832335
CCCAAAG76450.038.944641
GTAGCTG53350.038.6908386
AAAGTGC61150.038.2315064
ACTCGGG15300.037.7400324
GGAGGCT86300.037.406089
CCAAAGT71150.036.88442
AAGTGCT63600.036.5368355
TCGGGAG17950.035.543846
AGTGCTG67050.035.473096
Produced by FastQC (version 0.11.5)