FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763199

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763199
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3381743
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG10650.045.0932663
CCGAGTA11150.044.368182
GAGTAGC27600.041.4532284
AGTAGCT45050.039.714585
CTACTCG7050.038.7610972
CCCGAGT13100.038.535051
TACTCGG6650.038.232553
GTAGCTG47650.037.985916
GCTGGGA84050.037.403659
TAGCTGG50250.036.4715427
GCTACTC20350.033.3842431
CCCAAAG58750.033.0851251
TTGGGAG64050.032.2233056
AAAGTGC47650.031.8166244
CGTCGAG1201.5998376E-731.4522841
CGTACCG600.004126466331.3884533
CCAAAGT54300.030.7157142
GGAGGCT58000.030.706969
AAGTGCT51050.030.0665115
CGGAGGT12400.029.5595977
Produced by FastQC (version 0.11.5)