FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763200

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763200
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3381743
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG11000.050.3609432
TACTCGG10950.050.15923
CCGAGTA10550.044.054192
GCTACTC27600.043.3643571
CGAGTAG10600.042.9580653
GCTGGGA84750.041.9018339
ACGATCG450.001024012141.6964048
TCGGGAG14300.040.999656
GAGTAGC26450.039.756454
CCCGAGT13050.039.718281
GGAGGCT71550.038.7484789
CCCAAAG63550.038.3339231
AGTAGCT44350.038.31725
CTCGGGA14550.038.0713125
TTGGGAG68650.037.8508266
ACTCGGG14150.037.8210564
CGGAGGT16650.037.7515877
AAAGTGC51800.037.5193944
GTAGCTG45350.037.336716
TAGCTGG48550.036.8112377
Produced by FastQC (version 0.11.5)