FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763201

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763201
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3730737
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCGA2200.040.7400551
CGTCGAG2550.037.3788032
CGAGTAG12450.030.5841983
CCGAGTA12850.029.6703342
GCTGGGA99300.026.124449
GAGTAGC32600.025.7328554
AGTAGCT53400.024.399975
GTAGCTG55550.023.8840086
TAGCTGG63050.022.364917
CCCGAGT20200.021.5935731
CCCAAAG79000.020.0434191
AAAGTGC65500.019.9833284
AAGTGCT69200.019.6886465
CTAATCG1856.9025496E-419.3207992
GTGCTGG66650.019.2822427
GGGAGGC81150.019.1364658
TTGGGAG67950.019.0876966
AGTGCTG70500.018.566076
CCAAAGT75300.018.5125582
CTACTCG10650.018.4590742
Produced by FastQC (version 0.11.5)