FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763209

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763209
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33359910
Sequences flagged as poor quality0
Sequence length100-125
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTCC253950.037.058118-119
TCTACGC439100.036.079098118-119
CTACGCA421750.035.858112118-119
CGCGTAT49450.034.9459444-45
TACGCAC398750.034.52519116-117
ATCTACG471100.033.45392118-119
ATCGCGT53700.033.3536742-43
ACGCACA454700.030.322311118-119
CACCGTC330750.029.571396118-119
CGCACAG473900.029.050447118-119
TATGCCG244850.027.51824448-49
ATCGGAA430900.027.0580042
GATCGGA433950.027.0042381
CATCTAC608300.026.95565118-119
AGCCTAT273900.026.8368434-35
CCTATCA270500.026.8247436-37
ACATCTA626500.026.697344118-119
CGTCCTG304950.026.682793118-119
CCGTCCT323500.026.550135118-119
TCCACCG380600.025.806185118-119
Produced by FastQC (version 0.11.5)