FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763222

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763222
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32817355
Sequences flagged as poor quality0
Sequence length100-125
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCGT601850.053.217579
AAGAGCG653600.049.18128
ATCGGAA673600.047.901183
GATCGGA695000.046.514112
TCGGAAG697600.046.1079064
AGATCGG744350.043.4736251
CGGAAGA743000.043.2644925
CTTACGT383400.041.15443436-37
CGCCGTA205450.039.0101258-59
TTACGTG396850.038.6361538-39
TACGTGT411300.038.53449638-39
ACGTGTA413100.037.40884440-41
CCTTACG417900.036.68852636-37
GCCGTAT212450.036.6699460-61
GCATAGA220600.036.560703118-119
CGTATCA217450.036.38169562-63
TCGACGT262350.035.791107118-119
TCTCGAC283250.035.571762118-119
CCGTATC222400.035.5393960-61
AGTCTCG345700.035.234943118-119
Produced by FastQC (version 0.11.5)