FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005763224

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005763224
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33714117
Sequences flagged as poor quality0
Sequence length100-125
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGAC244850.041.758858118-119
CTCGACG239200.040.6026118-119
TCGACGT229050.038.373924116-117
ACGTCAG230700.037.32222118-119
CGACGTC239800.035.264454116-117
GACGTCA253600.034.962517118-119
GTCTCGA295100.034.90862118-119
AGTCTCG304800.033.96556116-117
ACAAGTC413500.033.03144118-119
CGTCAGC272950.031.920393116-117
CTTGATC503050.030.564402118-119
TGATCAC497450.030.084255118-119
CACAAGT451150.029.877253118-119
CTCTTGA566400.029.815218118-119
TCTTGAT565700.029.716215118-119
CAAGTCT445250.029.237188118-119
TCACAAG480950.028.93182118-119
TTGATCA508450.028.576605118-119
GATCACA498750.027.847666116-117
GTCTCTT624100.027.469313118-119
Produced by FastQC (version 0.11.5)